Solid-Phase Peptide Synthesis is a method for chemically constructing a peptide chain by anchoring the first amino acid to an insoluble resin and adding subsequent amino acids in sequence.
Introduced by Bruce Merrifield in 1963 (Nobel Prize in Chemistry, 1984), SPPS is the dominant method for producing research peptides today. The growing chain is anchored to a polymer resin, allowing excess reagents and byproducts to be washed away between coupling cycles.
Fmoc and Boc are the two main protection strategies; Fmoc-SPPS is the more common in modern peptide manufacturing. After the full sequence is assembled, the peptide is cleaved from the resin and purified — typically by reversed-phase HPLC.
All compounds in our catalog are produced by SPPS. Purity after synthesis depends on coupling efficiency, side reactions, and the post-synthesis purification step. The HPLC measurement reported on each COA reflects the result of this process for the specific lot.